The Synthesis, Identification, and Kinetic Characterization of the Photoaffinity Label: 3-Azidopyridine Adenosine Dinucleotide: An Advanced Undergraduate Laboratory Experiment Appropriate for Organic, Biochemistry, or Bio-organic Chemistry

TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 2 Issue 5 (1997), S1430-4171(97)05146-0

The Synthesis, Identification, and Kinetic Characterization of the Photoaffinity Label: 3-Azidopyridine Adenosine Dinucleotide: An Advanced Undergraduate Laboratory Experiment Appropriate for Organic, Biochemistry, or Bio-organic Chemistry

Allan A. Gahr and Yuzhuo Li*

Department of Chemistry, Clarkson University, Potsdam NY 13699-5810

Published online: 26 November 1997

Abstract. We present here a complete photochemical experiment suitable for biochemistry, bio-organic, and organic chemistry laboratories. It provides experiences in chemical and enzymatic syntheses, spectroscopy (IR, NMR, UV), chromatography (TLC, GC-MS), and a simple enzyme kinetic study utilizing UV spectroscopy. The application of light energy to produce chemical changes has recently expanded beyond photography, lithography, and organic synthesis to include use in tumor phototherapy and as antiviral agents. Dyes and a variety of photoactive chemicals have demonstrated potential use as environmentally benign insecticides, herbicides, and fungicides. Nevertheless, the traditional undergraduate chemical laboratory curriculum provides little exposure to photochemistry.

Key Words: Laboratories and Demonstrations; photoaffinity labeling; photochemistry; photobiology; organic; bio-organic; biochemistry; NAD⁺; undergraduate laboratory; UV spectroscopy; enzyme kinetics

(*) Corresponding author. (E-mail: yuzhuoli@craft.clarkson.edu)

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