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The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 5 Issue 1 (2000) pp 24-26

Photodamaging Effects of Porphyrin in a Human Carcinoma Cell Line

M. Gabriela Alvarez, Mariángeles La Penna, E. Ines Yslas, Viviana Rivarola, and Edgardo N. Durantini*

Departamento de Biología Molecular, Departamento de Química y Física, Universidad Nacional de Río Cuarto, Agencia Postal Nro 3, Río Cuarto 5800, Argentina

Published online: 1 February 2000

Abstract. A convenient procedure to visualize the photodynamic effect of porphyrins on cell lines is shown. 5,10,15,20-Tetra(4- methoxyphenyl)porphyrin (TMP) was used as photosensitizer. The culture flasks bearing a Hep-2 cell line were incubated for 24 h with 1 mM of TMP. Under these conditions saturation of the TMP intracellular concentration is obtained. The irradiation of cell cultures for 30 min produces 90% cell mortality, while no toxicity was observed under dark conditions or under irradiation without TMP. This methodology can be used to demonstrate the photodynamic therapy (PDT) process in a laboratory experiment.

Key Words:  Laboratories and Demonstrations; biochemistry; biochemical analysis;photosensitizer; tumor; fluorescence; UV-vis spectroscopy; irradiation; photodamage

(*) Corresponding author. (E-mail: edurantini@exa.unrc.edu.ar)

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Issue date: February 1, 2000

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