Simultaneous Determination of Two Antibiotics in Tablets by Spectrophotometry and Principal Component Regression (PCR) Analysis. An Advanced Undergraduate Experiment Involving Chemometrics

TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 5 Issue 5 (2000) pp 236-241

Simultaneous Determination of Two Antibiotics in Tablets by Spectrophotometry and Principal Component Regression (PCR) Analysis. An Advanced Undergraduate Experiment Involving Chemometrics

María É. Ribone, Ariana P. Pagani, Héctor C. Goicoechea, and Alejandro C. Olivieri*

Departamento de Química Analítica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario (2000), Argentina, and Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje El Pozo, Santa Fe(3000), Argentina

Published online: 2 October 2000

Abstract. An advanced analytical chemistry laboratory experiment involving the simultaneous determination of the antibiotics sulfamethoxazole and trimethoprime in commercial tablets is described. It involves the following steps: (1) preparation of nine calibration mixtures and the recording of their absorption spectra in the region 200–320 nm, (2) dissolution of the tablet contents and the recording of spectrophotometrics data, and (3) processing the data with the multivariate calibration technique of principal component regression (PCR). The theory of PCR is discussed, and a Visual Basic program is made available for data processing. The latter program allows students to obtain and save relevant statistical information (root-mean-square deviation, correlation coefficient, and relative error of prediction), as well as calibrate spectral factors and spectral residuals for each test sample. This program helps to illustrate the PCR technique in detail. The reagents used are of low cost and nontoxic; the experiment is simple and provides students with insight into a the real practice of integrating chemistry, instrumentation and computer techniques.

Key Words: Laboratories and Demonstrations; analytical chemistry

(*) Corresponding author. (E-mail: aolivier@fbioyf.unr.edu.ar)

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Supporting Materials:

Program (Zip file 3.31MB) 10.1007/s00897000417b