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The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 5 Issue 6 (2000) pp 324-328

Size-Exclusion Chromatography Using Dual Detection

Enrique Saiz and M. Pilar Tarazona*

Departamento de Química Física, Universidad de Alcalá, 28871 – Alcalá de Henares. Spain

Published online: 9 October 2000

Abstract. Size-exclusion chromatography (SEC) with dual detection, a differential refractometer as concentration detector and a multiangle laser light scattering as a molecular weight detector, is the basis for an experiment designed for students of polymer science. A brief description of the technique is included. The advantages of this technique over more traditional SEC with just one detector, which requires calibration with monodisperse standard polymers, are emphasized. Students determine molecular-weight distributions of easily synthesized polymers (or commercial samples), such as poly(methylmethacrylate) or polystyrene, and learn to interpret the results, to deal with commercially available software, and to visually complement the ideas taught in the polymer course lectures.

Key Words:  Laboratories and Demonstrations; analytic chemistry , polymer characterization, molecular-weight distributions, size-exclusion chromatography, light scattering

(*) Corresponding author. (E-mail:

Article in PDF format (247 KB) HTML format

Issue date: December 1, 2000

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