TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 7 Issue 1 (2002) pp 23-26

Capillary Electrophoresis of Water-Soluble Vitamins: An Undergraduate Experiment

Victòria Salvadó and Juan M. Sánchez*

Department of Chemistry, Universitat de Girona, Campus Montilivi s/n, 17071-Girona, Spain
Received October 18, 2001. Accepted December 4, 2001

Published online: 1 February 2002

Abstract. Capillary electrophoresis (CE) is a relatively new analytical separation technique that is not usually introduced in the undergraduate analytical chemistry curriculum. The technique’s growing popularity in research, industrial, and commercial laboratories, however, should be a reason to consider its introduction at this level. Here, we describe an exercise utilizing capillary zone electrophoresis and micellar electrokinetic chromatography. This exercise provides a suitable introduction to capillary electrophoresis and illustrates the mechanism for the separation of ionized and nonionized water-soluble vitamins (B1, B2 phosphate, B3 niacinamide, and B12). Joule heating can also be easily introduced as part of the exercise.

Key Words:  Laboratories and Demonstrations; organic chemistry; separation techniques; capillary electrophoresis

(*) Corresponding author. (E-mail: juanma.sanchez@udg.es)

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Issue date: February 1, 2002

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