The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 10
Issue 1 (2005) pp 26-28
Testing Human Saliva for Enzyme Activity: Use of an In-Class Kinetic Experiment for Nonscience Majors in a Large Classroom Setting
Marjorie A. Jones* and Christopher L. Cooper
Department of Chemistry, Illinois State University,
IL 61790-4160, firstname.lastname@example.org
Published online: 18 January 2005
Abstract. Nonscience students participated in an in-class experiment using an enzyme in their saliva known as amylase. The enzyme reacts with the high-molecular-weight glucose polymer starch, releasing small-molecular-weight oligosaccharides. Because high-molecular-weight starch and iodine yield a blue color, the loss of color indicated enzyme activity. Students reported that this inexpensive experiment increased their understanding of enzymes.
Key Words: Laboratories and Demonstrations; biochemistry; nonscience majors; enzyme kinetics; human saliva; amylase
(*) Corresponding author. (E-mail: email@example.com)
Supporting Materials:A student consent form and survey form for this experiment are available in a Zip file (44 KB).
Issue date: February