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The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 10 Issue 5 (2005) pp 447-451

Enzyme Kinetics of Catalase—An Introductory Chemistry Laboratory

Matthew S. Gibson, Brandon J. Cruickshank,* and John K. Nauman

Department of Chemistry, Northern Arizona University, Flagstaff, AZ 86011-5698,
Received June 17, 2005. Accepted August 25, 2005.

Published online: 21 Novenber 2005

Abstract. An introductory chemistry laboratory designed for an advanced high school or a college environment is presented. The experiment uses the enzyme catalase to catalyze the breakdown of hydrogen peroxide. The experiment covers important concepts including reaction rates, order of reaction, oxidation–reduction, plotting and analyzing data, and enzyme catalysis. This experiment was developed as part of a continuing curriculum reform effort in our introductory chemistry laboratories to integrate biological and chemical concepts, to reduce "cookbook" procedures, and to foster greater student engagement in the learning process.

Key Words: Laboratories and Demonstrations; general chemistry; kinetics; enzyme

(*) Corresponding author. (E-mail:

Article in PDF format (245 KB) HTML format

Supporting Materials:


Supporting Materials:

The experimental handout and pre-laboratory questions are available in a Zip file (110 KB).

Issue date: December 1, 2005

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