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The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 12 Issue 5 (2007) pp 343-346

Demonstration of Absorbance Using a Laptop Computer for Illumination, Webcam Image Capture and Image Analysis

Kyle D. Perry, James D. Landmark and Douglas F. Stickle*

Department of Pathology & Microbiology, University of Nebraska Medical Center, Omaha, NE 68198,
Received March 4, 2007. Accepted July 7, 2007.

Published online: 10 September 2007

Abstract. An experiment using a laptop computer with webcam color image analysis to demonstrate simple absorbance is described. The computer screen provides the illumination source. Image analysis parsing the blue color component gives a linear absorbance curve for aqueous serial dilutions of yellow food coloring. The demonstration is intended for high school chemistry laboratories where spectrophotometers are not generally available.

Key Words: Laboratories and Demonstrations; organic general; photometry; absorbance; computers

(*) Corresponding author. (E-mail:

Article in PDF format (155 KB) HTML format

Issue date: October 1, 2007

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