Qualitative Analysis of Several Amino Acids by COSY and DEPT Using Low-Field NMR: An Undergraduate Biochemistry or Instrumental Methods Laboratory Exercise

TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 13 Issue 5 (2008) pp 307-308

Qualitative Analysis of Several Amino Acids by COSY and DEPT Using Low-Field NMR: An Undergraduate Biochemistry or Instrumental Methods Laboratory Exercise

Michelle M. Ivey and Eugene T. Smith*

Harriet L. Wilkes Honors College, Florida Atlantic University, Jupiter, FL 33458, esmith@fau.edu
Received January 23, 2008. Accepted July 17, 2008.

Published online: 12 August 2008

Abstract. Low-field NMR is an inexpensive tool that can be used in teaching laboratories to illustrate fundamentals involved in obtaining and interpreting NMR spectra. In this exercise, a number of amino acids are identified from information provided in 1-D proton, COSY, and DEPT spectra. This laboratory exercise can be used in an undergraduate course in biochemistry or instrumental methods.

Key Words: Laboratories and Demonstrations; qualitative analysis, NMR

(*) Corresponding author. (E-mail: esmith@fau.edu)

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Supporting Materials:

Instructor notes, a sample student handout, and a sample Excel workbook are available online. The supplements were prepared with Microsoft Word and Excel (2003 SP2). Answers to the sample questions in the Student Handout are provided at the end of the Instructor Notes (192 KB).