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The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 14 Issue 5 (2009) pp 212-218

Quantitative Determination of Co-Eluting Compounds by Using Extract Ion Chromatogram in GC–MS

Chang Ji*, Jeffrey L. Dean, Rebecca A. Flores, and Susanne M. Boisvert

Department of Chemistry and Biochemistry, Texas State University – San Marcos, San Marcos, TX 78666, USA,
Received August 5, 2008. Accepted June 26, 2009

Published online: 29 December 2009

Abstract. An experiment for the quantitative determination of two co-eluting isomers, which are 3-pentanone and valeraldehyde, by using extract ion chromatogram in gas chromatograph–mass spectrometry (GC–MS) is described. The application is carried out by the internal standard method and the importance of molecular ion fragmentation via McLafferty rearrangement is emphasized. The unknown concentrations are readily calculated from the calibration curves generated by the students and compared with the actual data. The powerful capability of extract ion chromatogram in chemical analysis is appropriately demonstrated by this experiment, which can be fit into one session of the undergraduate instrumental analysis laboratory or other related scientific classes.

Key Words: Laboratories and Demonstrations; physical chemistry; organic chemistry

(*) Corresponding author. (E-mail:

Article in PDF format (36 KB) HTML format

Supporting Materials:

Laboratory handout for the students, notes for the instructor, as well as representative chromatograms and mass spectra are available online (68 KB).

Issue date: Dec, 31, 2009

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