A Deep Look at the Effect of Chromogenic Reagent Absorption on the Spectrophotometric Method

TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 15 (2010) pp 415-416

A Deep Look at the Effect of Chromogenic Reagent Absorption on the Spectrophotometric Method

Sun Feifei

School of Science, Tianjin University, Tianjin, 300072; P. R. China, sff@tju.edu.cn
Received September 9, 2009. Accepted March 17, 2010.

Published: 22 December 2010

Abstract. Some students have the misconception that monitoring the absorbance of a complex at a wavelength where the free ligand also absorbs strongly willdecrease the accuracy of the spectrophotometric method. This article gives the theoretical basis to show that ligand absorption can be corrected by a reagent blank and clarifies the effect of the undesirable chromogenic reagent absorption.

Key Words: In the Classroom; analytical chemistry; instrumental analysis; UV-Vis spectrophotometry; active learning

(*) Corresponding author. (E-mail: sff@tju.edu.cn)

Article in PDF format (127 KB) HTML format