The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 16
(2011) pp 109-113
A Model for the Teaching of Column Separation Science
Peter Peng Foo Lee,Yong Chua Teo, Timothy Ter Ming Tan*, Kelvin Jin Tai and Kelvin Jin Tai Koh
National Institute of Education, Nanyang Technological
University, 1 Nanyang Walk, Singapore 637616, Republic of Singapore,
Published: 25 March 2011
Abstract. The aim of this project is to develop a simple system that can be used to demonstrate the concepts and techniques of column chromatography to students from high school to university level. The system uses commonly available materials and apparatus, and complete experiments can be carried out within the timeframe of school laboratory-based lessons. A simple, cheap and reliable way to synthesize agarose microbeads for use as the stationary phase in a column is described. Mixture of dyes are also identified and optimized so that they separate cleanly into distinct bands in the column, and are therefore suitable for demonstration and student-led experimentation purposes.
Key Words: Laboratories and Demonstrations; general chemistry; separation science; classroom teaching; low-cost; column chromatography; dyes
(*) Corresponding author. (E-mail: firstname.lastname@example.org)
Supporting Materials:Time-lapse video of the separation can be freely downloaded from http://www.nsse.nie.edu.sg/ ~ttmtan/column/ for teaching and demonstration purposes (974 KB).