The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 18
(2013) pp 107-109
Identification of Unknown Proteins Using Three Forms of Polyacrylamide Gel Electrophoresis: A Laboratory Experiment
Rosa V. Flores* and Sherla Centeno
Department of Chemistry, University of Puerto Rico, Río Piedras Campus, San Juan, PR 00931-3346, email@example.com
Published: 17 May 2013
Abstract. A laboratory experiment involving a comparison between various forms of polyacrylamide gel electrophoresis (native, reducing and non-reducing sodium dodecyl sulfate) was performed in an undergraduate biochemistry laboratory. Students executed one form of electrophoresis, and analyzed the same seven unknown samples using both fixed (10%) and gradient (4–20%) polyacrylamide gels. Based on the key protein properties of sixteen unknowns, together with the combined student results and focus questions that guided interpretation of gel data, students identified the unknown protein samples and gained valuable experience on each form of electrophoresis, while contrasting fixed and gradient polyacrylamide gel separations.
Key Words: Laboratories and Demonstrations; biochemistry; polyacrylamide gel electrophoresis; proteins; Native-PAGE; Reducing SDS-PAGE; Non-reducing SDS-PAGE; experimental learning
(*) Corresponding author. (E-mail: firstname.lastname@example.org )
Supporting Materials:One supporting file is available, General Information for Advanced Organic Chemistr (359 KB).