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The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 18
(2013) pp 165-172
DOI 10.1007/s00897132491
Excitation-Emission Fluorescence Spectroscopic Analysis in the Presence of Interferents: A Laboratory Experiment Integrating Analytical Chemistry and Advanced Data Processing
Valeria Boeris, Miguel A. Cabezón and Alejandro C. Olivieri*
Departamento de Química Analítica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario e Instituto de Química Rosario (IQUIR-CONICET), Suipacha 531, Rosario (2000), Argentina, aolivier@fbioyf.unr.edu.ar
Received January 5, 2013. Accepted May 18, 2013.
Published: 5 July 2013
Abstract. In the present report an advanced experiment is described to introduce chemistry students to the direct chemometric resolution of binary mixtures of two pharmaceuticals (oxatomide and phenylephrine) by measuring excitation-emission fluorescence matrices, and processing them with second-order calibration based on a decomposition algorithm called parallel factor analysis (PARAFAC). This experiment is of interest because it combines experimental analytical chemistry (preparation of standard solutions, spectrofluorimetric measurements, etc.) with computer applications not requiring deep programming skills. Second-order multivariate calibration was implemented with a simple graphical toolbox especially designed for this report: mvc2basic, which is available as supplementary material. This work provides students the opportunity to be introduced to advanced chemometrics activities in an accessible manner.
Key Words: Laboratories and Demonstrations; analytical chemistry
(*) Corresponding author. (E-mail: aolivier@fbioyf.unr.edu.ar)
Article in PDF format (778 KB) HTML format
Supporting Materials:
A zip file containing mvc2basic_manual, mvc2basic_program, and mvc2basic_simulated_example files are available (284 KB).
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