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The Chemical Educator

ISSN: 1430-4171 (electronic version)

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Abstract Volume 20 (2015) pp 9-12

Identification of Disaccharides Using HPLC: An Undergraduate Experiment

Wendell Black, Jon Villareal, Tammy Melton, and Paul Kline*

Department of Chemistry, Middle Tennessee State University, Murfreesboro, TN 37132,
Received July 8, 2014. Accepted November 18, 2014.

Published: 30 January 2015

Abstract. Disaccharides, one of the most important groups of biomolecules, are often given little attention in undergraduate biochemistry laboratories. Due to their closely related structures it is often difficult to distinguish experimentally between monosaccharides. An undergraduate experiment for the biochemistry laboratory is detailed in which students identify a disaccharide among a group of four, based on the results of Benedict’s test, reactivity with an enzyme, and identification of the monosaccharides present using an HPLC equipped with a charged aerosol detector.

Key Words: Laboratories and Demonstrations; biochemistry

(*) Corresponding author. (E-mail:

Article in PDF format (607 KB) HTML format

Supporting Materials:

The following information is available as supporting material: (i) Student handout; (ii) Instructors briefing notes and experimental preparation. (211 KB).

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