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The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 20
(2015) pp 285-288
Analysis of an Alpha Amino Acid by Fourier Transform Nuclear Magnetic Resonance Spectrometry: Identification and Titration
Christina M. Kraemer-Chant* and Alayne L. Schroll
Department of Chemistry and Physics, Saint Michael’s College, Colchester, Vermont 05439, cchant@smcvt.edu
Received August 1, 2015. Accepted September 23, 2015.
Published: 14 October 2015
Abstract. This paper outlines an undergraduate biochemistry laboratory project involving the identification and titration of an alpha amino acid by Fourier transform nuclear magnetic resonance spectrometry (FT NMR). This project is carried out early in the semester and is useful for reviewing and giving the students more extensive hands-on use of the 1H NMR. It also serves to lay the groundwork for more advanced NMR projects and to reinforce the concepts of acid/base chemistry and titration as they relate to amino acids, peptides, and proteins. All of these concepts are being covered simultaneously in the lecture part of the course.
Key Words: Laboratories and Demonstrations; biochemistry, hands-on learning, amino acids, NMR spectroscopy
(*) Corresponding author. (E-mail: cchant@smcvt.edu)
Article in PDF format (141 KB) HTML format
Supporting Materials:
The supporting information includes: a) The lab handout issued to the students; b) Notes to the lab instructor; c) A special preparative note; and d) CAS numbers for all the chemicals. (12 KB)
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