Mass Sensitive and Electrochemical Detection of “Chloramphenicol” by Molecularly Imprinted Polymer and Molecular Imprinted Polymer Beads Utilizing Electrochemical Instruments

TCE

The Chemical Educator

ISSN: 1430-4171 (electronic version)

Table of Contents

Abstract Volume 25 (2020) pp 155-158

Mass Sensitive and Electrochemical Detection of “Chloramphenicol” by Molecularly Imprinted Polymer and Molecular Imprinted Polymer Beads Utilizing Electrochemical Instruments

Aysha Shaheen^†, Dionysios (Dion) D. Dionysiou^‡, Sadia Z. Bajwa^† and Suzanne Lunsford *^,§

^†Nanobiotech Group, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box No.577, Jhang Road, Faisalabad, Pakistan; ^‡Environmental Engineering and Science Program, Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, OH 45221, United States; ^§Wright State University, Department of Chemistry, 3640 Colonel Glenn Highway, Dayton, OH 45435 suzanne.lunsford@wright.edu Received May 27, 2020. Accepted August 11, 2020.

Published: 26 August 2020

Abstract. In this article, we describe an inquiry-based learning experience with detection of “Chloramphenicol” (CAP)usingmolecularly imprinted polymer (MIP) and molecularly imprinted polymer beads (MIP beads) modified glassy carbon electrodes. Two different approaches were utilized to estimate the potential of the MIP and MIP Beads towards CAP detection, one is electrochemical using Cyclic voltammetry (CV) and capacitance measurement using LCR meter and the other is mass sensitive detection using “Quartz Crystal Microbalance”. Atomic Force Microscopy (AFM) was utilized to analyze the morphology of the MIP beads. Modified electrodes for respective instruments were challenged with the increasing concentrations of CAPand they displayed significant response towards these incremental concentrations suggesting that these two approaches with three different instruments can be successfully used for the detection of small molecules like CAP with greater sensitivity and selectivity.

Key Words: Laboratories and Demonstrations; analytic chemistry; inquiry-based experiment; cyclic voltammetry (CV); quartz Crystal microbalance (QCM); LCR meter; modified glassy carbon electrode; quartz crystal; Gold printed chip; molecularly imprinted polymer (MIP)

(*) Corresponding author. (E-mail: suzanne.lunsford@wright.edu )

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