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The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 28
(2023) pp 1-5
HPLC Analysis of Antioxidants in Tea: A Project/Inquiry-based
Quantitative Analysis Laboratory Experiment
Andrew Justl, Steven Landeweer, Xiaohan Yuan and Manori Perera*
Department of Chemistry, 201 E. Beecher Street, Illinois
Wesleyan University, Bloomington, IL 61701, mperera@iwu.edu
Received July 12, 2020. Accepted November 18, 2020.
Published: 18 January 2023
Abstract. An inquiry-based lab was created to
measure and analyze the antioxidant contents in different teas using
high-performance liquid chromatography (HPLC) over two 4-hour periods for
second- or third-year chemistry students. Students become conversant with the
instrument’s capacity thru method devolvement. They also learn about sample
preparation, calibration methods, identification, and quantification of an
unknown through measurements of antioxidants. A project/inquiry-based lab
offers insight into independent research with more opportunities for creativity
than traditional labs where students are guided with procedural steps.
Key Words: Laboratories and Demonstrations; Project/inquiry based experiment; analytical chemistry; instrumentation with HPLC; mini-research on tea antioxidant
(*) Corresponding author. (E-mail: mperera@iwu.edu)
Article in PDF formatt(201 KB) HTML fomat
Supporting Materials:
A PDF containing
the Chromatograms of common antioxidants found in tea was provided to the
students so they could identify the popular antioxidants in their
chromatograms.( 106 KB)
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