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The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 28
(2023) pp 127-134
ELISA Assay with PDMS Microfluidic Channels Fabricated by 3D Printed Master Mold
Virginia R. Greenberger, Hannah Yang, Tinglu S. Yang, Angela M. Bischof, Paul S. Cremer, Dan G. Sykes*
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA, dgs12@psu.edu
Received July 25, 2023. Accepted October 2, 2023.
Published: 17 October 2023
Abstract. Following the emergence of the SARS-CoV-2 (Covid-19) pandemic, interest in understanding antibody diagnostic testing has increased. We describe a quick and inexpensive technique that enabled students to print their own microfluidic devices that can be used to house an immunoassay for detecting a Human Immunodeficiency Virus (HIV) antibody. Both qualitative diagnostic assays and quantitative binding assays were carried out to characterize the HIV interaction with a target antibody. By performing these hands-on low-cost experiments in the analytical chemistry lab course, students were exposed to 3D fabrication, microfluidic technology, surface chemistry, protein-ligand binding affinity studies, and immunoassays within the time frame of two four–hour laboratory periods.
Key Words: Laboratories and Demonstrations; analytical chemistry; laboratory instruction; interdisciplinary; multidisciplinary; hands-on learning; bioanalytical chemistry; microscale lab;
(*) Corresponding author.
(E-mail:
Article in PDF formatt(695 KB) HTML format
Supporting Materials:
Included are: Instructor Notes and Student Handout, Grading Rubric, Example Student Lab Report, Student Reflections, Excel File with ELISA Fitting Macro, as well as Source stl, dwg, and gcode files. (1309 KB)
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