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The Chemical Educator
ISSN: 1430-4171 (electronic version)
Abstract Volume 2
Issue 6 (1997), S1430-4171(97)06151-4
DOI 10.1333/s00897970151a [old
prefix 10.1007/]
Two-Dimensional High-Performance Liquid Chromatography
Frantisek Svec
Department of Chemistry, University of California, Berkeley, CA 94720-1460
Published online: 24 December 1997
Abstract. The quest for a substantial increase in the peak capacity and, therefore, in the number of compounds that can be separated in a single chromatographic run, led to the development of multidimensional techniques in high-performance liquid chromatography (HPLC). The "classical" method of collecting fractions from the initial separation and separating them further in the secondary steps is very powerful but its fully automated "online" version requires quite complex instrumentation. In contrast, development of new chromatographic media with segregated bimodal chemistries allows the 2-D separations to be achieved in a single column with standard HPLC equipment.
Key Words: In the Classroom; chromatography; HPLC; separation media; pore-size-specific modification; direct injection; instrumentation; two-dimensional separation
(*) Corresponding author. (E-mail: svec@uclink4.berkeley.edu)
Article in PDF format (198 KB ) HTML format
Issue date: December
24, 1997
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